Isolation of bacteria from soft coral Sinularia spp. and test of their antibiotic resistance on Tetracyline, Gentamicin and Cefazolin

Abstract

Soft coral is marine organism considered as a source of bioactive compounds. However, the relationship between soft coral, its associated microorganisms and its bioactive compounds are still discussed. Four among five collected specimens of soft coral were identified as Sinularia gibberosa Tixier-Durivault, 1970 (code: SH1); Sinularia maxima Verseveldt, 1971 (code: SH2); Sinularira capillosa Tixier-Durivault, 1970 (code: SH3); and Sinularia mira Tixier-Durivault, 1970 (code: SH4). 28 bacteria strains were isolated from specimens of soft corals by traditional culture method, including 26 strains of Firmicutes phylum and two others of Proteobacteria phylum. Among 25 strains tested for antibiotic activity, only the KH4 exhibited resistance to Cefazoline, then this strain was identified as Bacillus cereus 1 by using the API 50CHB KIT with accuracy of 99.7%. The other strains showed the sensitivity to Cefazolin with inhibitable zone ranging from 6 to 17mm. Strains of KH6 and KH10 showed the weak sensitivity with Gentamicine and Cefazolin, while KH18 showed the weak sensitivity to Tetracyline.